HPLC Column, HPS Silica, 5um, 4.6mm ID x 100mm Length, 120A. Cogent Brand. 1 EA.

Additional Info:

75001-10P Cogent HPS Silica™ HPLC Column is packed with high‑purity, unmodified silica, delivering consistent and reproducible performance for normal phase chromatography and USP L3 classified methods.

Because the stationary phase is not chemically bonded, it provides strong, direct interactions with polar and hydrophilic compounds, making it highly effective for fats, lipids, and other polar analytes.

This proprietary HPS Silica™ has been trusted by laboratories for many years as a premium, cost‑effective alternative to established silica products such as Inertsil® and Luna®, delivering dependable selectivity and long‑term reproducibility.

The 5 µm particle size offers a balance of efficiency and durability, allowing higher flow rates with lower system backpressure, making it ideal for routine analytical use on standard HPLC systems.

100 mm Length – Balanced Resolution for Normal Phase HPLC

The 100 mm column length provides increased resolving power without excessive run times.

  • Greater resolution than 50 mm columns

  • Improved separation of closely related compounds

  • Efficient run times for routine and QC workflows

✅ Recommended for applications where moderate resolution improvement is needed

4.6 mm ID – Standard Analytical Format

  • Designed for 4.6 mm ID analytical columns

  • Compatible with standard HPLC systems

  • Higher sample loading capacity

  • Ideal for routine analytical and QC methods

✅ Recommended for general-purpose HPLC and compendial methods

Flow Rate & System Requirements (4.6 mm ID, 5 µm)

✅ Compatible with standard HPLC systems

  • Typical flow rate range: ~1.0 – 1.5 mL/min

  • Lower backpressure compared to smaller particle sizes

  • Suitable for routine analytical separations

  • Compatible with UV and other standard detectors

✅ Best Practice: Optimize mobile phase composition for consistent normal phase retention

Why Choose HPS Silica™ Columns

High‑Purity Unmodified Silica

  • Ideal for normal phase chromatography

  • Strong retention of polar compounds

USP L3 Compatibility

  • Suitable for compendial methods

  • Reliable alternative to legacy silica columns

Reproducible Performance

  • Consistent selectivity

  • Proven reliability in long‑term use

Performance Advantages

Lower Backpressure (5 µm Particles)

  • Enables higher flow rates

  • Compatible with conventional systems

Fast Equilibration

  • Reduces analysis cycle time

Effective Polar Retention

  • Ideal for lipids, fats, and hydrophilic analytes

Typical Applications

  • Fats and lipid analysis

  • Normal phase HPLC separations

  • Polar compound analysis

  • USP L3 analytical methods

  • Food, pharmaceutical, and chemical testing

Important Usage Notes

  • Designed for normal phase chromatography conditions

  • Use appropriate non‑polar mobile phases

  • Ensure adequate equilibration for reproducible retention

  • Follow standard silica handling and storage procedures

 

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